Faculty Profiles

 
Dr. Kashif Bashir

Dr. Kashif Bashir





Assistant Professor
Department of Biological Sciences (Sargodha)
Profile Viewed : 286

Biography:


My specific interest is in Molecular Biology, Molecular Genetics, Cancer Genetics, DNA Repair pathways and Mutagenesis, Tumour cell Culture and Gene Knock out models, Endocrinology and LAB. Skills includes, Cancer cell lines cell culturing, cloning, genetic engineering, Preparation of knockdown model using RNAi (shRNA) approach, DNA extraction from blood by manual and kit method, RNA extraction from tissue by manual and kit method, Genotyping techniques using ARMS PCR, RFLP, Expressional Analysis using Real Time PCR and Immunohistochemistry Techniques

LAB. Skills.
1. Cancer cell lines cell culturing, cloning, genetic engineering (worked on thyroid cancer cell lines)
2. Preparation of knockdown model using RNAi (shRNA) approach (worked on thyroid cancer cell lines)
3. DNA extraction from blood by manual and kit method (worked on thyroid cancer cell lines)
4. RNA extraction from tissue by manual and kit method.
5. Genotyping techniques using ARMS PCR, RFLP (worked on thyroid cancer cell lines)
6. Expressional Analysis using Real Time PCR (worked on thyroid cancer cell lines)
7. Immunohistochemistry Techniques (worked on thyroid cancer cell lines)
RESEARCH INTERESTS
Molecular Biology, Molecular Genetics, Cancer Genetics, DNA Repair and Mutagenesis, Tumor cell Culture and Gene Knock out models, Endocrinology